Journal: Scientific Reports

Article Title: Effect of lysine side chain length on histone lysine acetyltransferase catalysis

doi: 10.1038/s41598-020-69510-0

Figure Lengend Snippet: Synthesis and enzymatic evaluation of lysine analogs with altered chain length. ( a ) Synthetic scheme for the preparation of lysine analogs with longer chain length; ( b ) Graphic representation of AcCoA-mediated acetylation of H4 peptides by MOF. ( c ) Graphic representation of AcCoA-mediated acetylation of H3 peptides by PCAF and GCN5.

Article Snippet: Plasmid carrying recombinant His-tagged Human GCN5 catalytic domain (residues 497–662 in pET28a-LIC vector) was purchased from Addgene (25,482).

Techniques:

Journal: Scientific Reports

Article Title: Effect of lysine side chain length on histone lysine acetyltransferase catalysis

doi: 10.1038/s41598-020-69510-0

Figure Lengend Snippet: Kinetics parameters for KAT-catalyzed acetylation of H4K*16, H3K*14 and H3K*9 histone peptides.

Article Snippet: Plasmid carrying recombinant His-tagged Human GCN5 catalytic domain (residues 497–662 in pET28a-LIC vector) was purchased from Addgene (25,482).

Techniques:

Journal: Scientific Reports

Article Title: Effect of lysine side chain length on histone lysine acetyltransferase catalysis

doi: 10.1038/s41598-020-69510-0

Figure Lengend Snippet: Enzymes’ residual activity upon incubation of MOF (0.2 µM), PCAF (0.5 µM) or GCN5 (0.5 µM) with H4K*16 (MOF) or H3K*14/H3K*9 (PCAF and GCN5) histone peptides.

Article Snippet: Plasmid carrying recombinant His-tagged Human GCN5 catalytic domain (residues 497–662 in pET28a-LIC vector) was purchased from Addgene (25,482).

Techniques: Activity Assay, Incubation

Journal:

Article Title: Regulated Expression of the Escherichia coli lepB Gene as a Tool for Cellular Testing of Antimicrobial Compounds That Inhibit Signal Peptidase I In Vitro

doi: 10.1128/AAC.46.11.3549-3554.2002

Figure Lengend Snippet: Susceptibility of a panel of microorganisms to SPase inhibitors

Article Snippet: MD1, MD2, and MD3 were selected from a high-throughput screen that utilized a biochemical assay. table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Microorganism MIC (μg/ml) MD1 MD2 MD3 E. coli ATCC 25922 >64 >11.4 >9 Haemophilus influenzae ATCC 49766 8 >11.4 9 Moraxella catarrhalis ATCC 25238 2 5.7 2.3 S. aureus ATCC 13709 2 11.4 4.5 Streptococcus pneumoniae ATCC 49619 ND a 11.4 >9 Candida albicans ATCC 90028 4 >11.4 9 Open in a separate window a ND, not determined.

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Journal:

Article Title: Development of a Whole-Cell Assay for Peptidoglycan Biosynthesis Inhibitors

doi: 10.1128/AAC.46.4.943-946.2002

Figure Lengend Snippet: MurA inactivation by Cpd1 following preincubation in the presence (closed circles) or absence (open circles) of 1 mM UDP-GlcNAc.

Article Snippet: Cpd1 inhibited growth of several microorganisms, although at 32.2 μg/ml no growth inhibition of E. coli was observed (Table ). table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Microorganism MIC (μg/ml) Cpd2 Cpd1 Escherichia coli ATCC 25922 >12.2 >32.3 Haemophilus influenzae ATCC 49766 >12.2 32.3 Moraxella catarrhalis ATCC 25238 >12.2 8.1 Staphylococcus aureus ATCC 13709 >12.2 16.1 Streptococcus pneumoniae ATCC 49619 >12.2 16.1 Candida albicans ATCC 90028 >12.2 32.3 Open in a separate window Susceptibility of a panel of microorganisms to MurA inhibitors

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Journal:

Article Title: Development of a Whole-Cell Assay for Peptidoglycan Biosynthesis Inhibitors

doi: 10.1128/AAC.46.4.943-946.2002

Figure Lengend Snippet: Time-dependent inactivation of MurA during preincubation with Cpd1 at 2.5 (▴), 5 (▵), 10 (○), 20 (•), or 30 (□) μM or with DMSO (▪). The compound was dissolved in 100% DMSO.

Article Snippet: Cpd1 inhibited growth of several microorganisms, although at 32.2 μg/ml no growth inhibition of E. coli was observed (Table ). table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Microorganism MIC (μg/ml) Cpd2 Cpd1 Escherichia coli ATCC 25922 >12.2 >32.3 Haemophilus influenzae ATCC 49766 >12.2 32.3 Moraxella catarrhalis ATCC 25238 >12.2 8.1 Staphylococcus aureus ATCC 13709 >12.2 16.1 Streptococcus pneumoniae ATCC 49619 >12.2 16.1 Candida albicans ATCC 90028 >12.2 32.3 Open in a separate window Susceptibility of a panel of microorganisms to MurA inhibitors

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Journal:

Article Title: Development of a Whole-Cell Assay for Peptidoglycan Biosynthesis Inhibitors

doi: 10.1128/AAC.46.4.943-946.2002

Figure Lengend Snippet: Susceptibility of a panel of microorganisms to MurA inhibitors

Article Snippet: Cpd1 inhibited growth of several microorganisms, although at 32.2 μg/ml no growth inhibition of E. coli was observed (Table ). table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Microorganism MIC (μg/ml) Cpd2 Cpd1 Escherichia coli ATCC 25922 >12.2 >32.3 Haemophilus influenzae ATCC 49766 >12.2 32.3 Moraxella catarrhalis ATCC 25238 >12.2 8.1 Staphylococcus aureus ATCC 13709 >12.2 16.1 Streptococcus pneumoniae ATCC 49619 >12.2 16.1 Candida albicans ATCC 90028 >12.2 32.3 Open in a separate window Susceptibility of a panel of microorganisms to MurA inhibitors

Techniques: